Simian immunodeficiency virus (SIV) -specific CDS+ cytotoxic T lymphocyte responses of naive and vaccinated cynomolgus macaques infected with SIVmac32H(JS): quantitative analysis by in vitro antigenic stimulation

Detailed analyses of simian immunodeficiency virus (SN)-specific cytotoxic T lymphocyte (CTL) responses in vaccinated and infected macaques may help to clarzfi the role of CTL immunity in protection against lentiviruses. Here, the optimal conditions for the measurement of SIV Gag-specific CTL were investigated by bulk and limiting dilution assays of peripheral blood mononuclear cells (PBMC) from naive and vaccinated cynomolgus macaques (Macaca fascicularis) infected with SIVmac32H(JS). In vitro restimulation tias generally required for CTL detection. Selective activation of CD8’ and MHC-restricted SIV Gag-specific CTL was induced by stimulation with autologous para-formaldehyde-Jixed B-lymphoblastoid cell lines infected with a recombinant vaccinia virus expressing SIV Gag. Applied to limiting dilution assays, antigenic stimulation reproducibly demonstrated SIV Gag-specific CTL precursors (CTLp) in PBMC of all animals studied, including those lacking significant responses in standard bulk CTL assays.